Probing formation of cargo/importin-α transport complexes in plant cells using a pathogen effector.

ABSTRACT: Importin-αs are essential adapter proteins that recruit cytoplasmic proteins destined for active nuclear import to the nuclear transport machinery. Cargo proteins interact with the importin-α armadillo repeat domain via nuclear localization sequences (NLSs), short amino acids motifs enriched in Lys and Arg residues. Plant genomes typically encode several importin-α paralogs that can have both specific and partially redundant functions. Although some cargos are preferentially imported by a distinct importin-α it remains unknown how this specificity is generated and to what extent cargos compete for binding to nuclear transport receptors. Here we report that the effector protein HaRxL106 from the oomycete pathogen Hyaloperonospora arabidopsidis co-opts the host cell's nuclear import machiner

SUBMITTER: Wirthmueller L 

PROVIDER: S-EPMC4350430 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature