Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering.

ABSTRACT:

Background

Over the last decades, molecular cloning has transformed biological sciences. Having profoundly impacted various areas such as basic science, clinical, pharmaceutical, and environmental fields, the use of recombinant DNA has successfully started to enter the field of cellular engineering. Here, the polymerase chain reaction (PCR) represents one of the most essential tools. Due to the emergence of novel and efficient PCR reagents, cloning kits, and software, there is a need for a concise and comprehensive protocol that explains all steps of PCR cloning starting from the primer design, performing PCR, sequencing PCR products, analysis of the sequencing data, and finally the assessment of gene expression. It is the aim of this methodology paper to provide a comprehensive pr

SUBMITTER: Hoseini SS 

PROVIDER: S-EPMC4350901 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature