Criteria for selecting microhaplotypes: mixture detection and deconvolution.

ABSTRACT:

Background

DNA sequencing is likely to become a standard typing method in forensics in the near future. We define a microhaplotype to be a locus with two or more single nucleotide polymorphisms (SNPs) that occur within a short segment of DNA (e.g., 200 bp) that can be covered by a single sequence run and collectively define a multiallelic locus. Microhaplotypes can be highly informative for many forensic questions, including detection of mixtures of two or more sources in a DNA sample, a common problem in forensic practice.

Results

When all alleles are equally frequent, the probability of detecting three or more alleles in a mixture is at maximum. The classical population genetics concept of effective number of alleles at a locus, termed Ae, converts the unequal allele frequ

SUBMITTER: Kidd KK 

PROVIDER: S-EPMC4351693 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature