Genome-wide, whole mount in situ analysis of transcriptional regulators in zebrafish embryos.

ABSTRACT: Transcription is the primary step in the retrieval of genetic information. A substantial proportion of the protein repertoire of each organism consists of transcriptional regulators (TRs). It is believed that the differential expression and combinatorial action of these TRs is essential for vertebrate development and body homeostasis. We mined the zebrafish genome exhaustively for genes encoding TRs and determined their expression in the zebrafish embryo by sequencing to saturation and in situ hybridisation. At the evolutionary conserved phylotypic stage, 75% of the 3302 TR genes encoded in the genome are already expressed. The number of expressed TR genes increases only marginally in subsequent stages and is maintained during adulthood suggesting important roles of the TR genes in body ho

SUBMITTER: Armant O 

PROVIDER: S-EPMC4351915 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature