ITIS, a bioinformatics tool for accurate identification of transposon insertion sites using next-generation sequencing data.

ABSTRACT:

Background

Transposable elements constitute an important part of the genome and are essential in adaptive mechanisms. Transposition events associated with phenotypic changes occur naturally or are induced in insertional mutant populations. Transposon mutagenesis results in multiple random insertions and recovery of most/all the insertions is critical for forward genetics study. Using genome next-generation sequencing data and appropriate bioinformatics tool, it is plausible to accurately identify transposon insertion sites, which could provide candidate causal mutations for desired phenotypes for further functional validation.

Results

We developed a novel bioinformatics tool, ITIS (Identification of Transposon Insertion Sites), for localizing transposon insertion sites withi

SUBMITTER: Jiang C 

PROVIDER: S-EPMC4351942 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature