Identification and characterization of VpsR and VpsT binding sites in Vibrio cholerae.

ABSTRACT:

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The ability to form biofilms is critical for environmental survival and transmission of Vibrio cholerae, a facultative human pathogen responsible for the disease cholera. Biofilm formation is controlled by several transcriptional regulators and alternative sigma factors. In this study, we report that the two main positive regulators of biofilm formation, VpsR and VpsT, bind to nonoverlapping target sequences in the regulatory region of vpsL in vitro. VpsR binds to a proximal site (the R1 box) as well as a distal site (the R2 box) with respect to the transcriptional start site identified upstream of vpsL. The VpsT binding site (the T box) is located between the R1 and R2 boxes. While mutations in the T and R boxes resulted in a decrease in vpsL expression, deletion of the

SUBMITTER: Zamorano-Sanchez D 

PROVIDER: S-EPMC4352665 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature