Designing novel and simple competitive internal amplification control for reliable PCR diagnosis of herpes simplex virus.

ABSTRACT:

Background

PCR is a molecular technique for herpes simplex virus (HSV) detection that can cause life-threatening infections such as encephalitis and keratitis. However, the main issues, false-negative results causing by PCR inhibitors, of this technique that reduce PCR efficiency. To overcome this problem, a competitive internal amplification control (IAC) was constructed for conventional PCR using the PCR-cloning technique.

Objectives

The purpose of this study is the design of competitive IAC for PCR diagnosis of HSV, which in fact is the main cause of keratitis and viral encephalitis in developed countries.

Materials and methods

Composite primers for PCR amplification of Leishmania major kDNA (kinetoplast DNA) were designed and optimized to use as IAC-HSV. IAC-HSV a

SUBMITTER: Akbarian A 

PROVIDER: S-EPMC4353061 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature