A manual small molecule screen approaching high-throughput using zebrafish embryos.

ABSTRACT: Zebrafish have become a widely used model organism to investigate the mechanisms that underlie developmental biology and to study human disease pathology due to their considerable degree of genetic conservation with humans. Chemical genetics entails testing the effect that small molecules have on a biological process and is becoming a popular translational research method to identify therapeutic compounds. Zebrafish are specifically appealing to use for chemical genetics because of their ability to produce large clutches of transparent embryos, which are externally fertilized. Furthermore, zebrafish embryos can be easily drug treated by the simple addition of a compound to the embryo media. Using whole-mount in situ hybridization (WISH), mRNA expression can be clearly visualized within zeb

SUBMITTER: Poureetezadi SJ 

PROVIDER: S-EPMC4353429 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature