Dermcidin exerts its oncogenic effects in breast cancer via modulation of ERBB signaling.

ABSTRACT:

Background

We previously identified dermicidin (DCD), which encodes a growth and survival factor, as a gene amplified and overexpressed in a subset of breast tumors. Patients with DCD-positive breast cancer have worse prognostic features. We therefore searched for specific molecular signatures in DCD-positive breast carcinomas from patients and representative cell lines.

Methods

DCD expression was evaluated by qRT-PCR, immunohistochemical and immunoblot assays in normal and neoplastic tissues and cell lines. To investigate the role of DCD in breast tumorigenesis, we analyzed the consequences of its downregulation in human breast cancer cell lines using three specific shRNA lentiviral vectors. Genes up- and down-regulated by DCD were identified using Affymetrix microarray and

SUBMITTER: Bancovik J 

PROVIDER: S-EPMC4353460 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature