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IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans.

ABSTRACT: RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology or environment.

SUBMITTER: Nagy AI

PROVIDER: S-EPMC4353841 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans.

Nagy Anikó I AI Vázquez-Manrique Rafael P RP Lopez Marie M Christov Christo P CP Sequedo María Dolores MD Herzog Mareike M Herlihy Anna E AE Bodak Maxime M Gatsi Roxani R Baylis Howard A HA

EMBO reports 20150121 3

RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivit ...[more]

PMID: 25608529

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