Deleting maternal Gtl2 leads to growth enhancement and decreased expression of stem cell markers in teratoma.

ABSTRACT: The distal region of mouse chromosome 12 harbors the Dlk1-Dio3 domain, is essential for normal development and encodes maternally expressed noncoding RNAs (ncRNAs), including Gtl2 as well as paternally expressed proteins.Gtl2 works as a tumor suppressor in several types of human cancer cell lines; however, whether this reflects its function in vivo is unknown. Deleting Gtl2 from the maternal allele (Gtl2((-/+))) results in loss of expression of Gtl2 and decreased expression of downstream ncRNAs, including many miRNAs. To determine the role of ncRNAs in tumorigenesis, we induced teratomas by engrafting E6.5 embryos (wildtype or Gtl2((-/+))) under the kidney capsule of scid mice. Some teratomas derived from the Gtl2((-/+)) embryos exhibited hypertrophic growth, suggesting that ncRNAs, includ

SUBMITTER: Takahashi N 

PROVIDER: S-EPMC4354225 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature