Four core genotypes mouse model: localization of the Sry transgene and bioassay for testicular hormone levels.

ABSTRACT:

Background

The "four core genotypes" (FCG) mouse model has emerged as a major model testing if sex differences in phenotypes are caused by sex chromosome complement (XX vs. XY) or gonadal hormones or both. The model involves deletion of the testis-determining gene Sry from the Y chromosome and insertion of an Sry transgene onto an autosome. It produces XX and XY mice with testes, and XX and XY mice with ovaries, so that XX and XY mice with the same type of gonad can be compared to assess phenotypic effects of sex chromosome complement in cells and tissues.

Findings

We used PCR to amplify the Sry transgene and adjacent genomic sequences, to resolve the location of the Sry transgene to chromosome 3 and confirmed this location by fluorescence in situ hybridization (FISH) of the

SUBMITTER: Itoh Y 

PROVIDER: S-EPMC4354741 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature