Proteomic analysis of immediate-early response plasma proteins after 70% and 90% partial hepatectomy.

ABSTRACT:

Aim

Partial hepatectomy (PH) induces robust hepatic regenerative and metabolic responses that are considered to be triggered by humoral factors. The aim of the study was to identify plasma protein factors that potentially trigger or reflect the body's immediate-early responses to liver mass reduction.

Methods

Male C57BL/6 mice were subjected to sham operation, 70% PH or 90% PH. Blood was collected from the inferior vena cava at 20, 60 and 180 min after surgery.

Results

Using a label-free quantitative mass spectrometry-based proteomics approach, we identified 399 proteins exhibiting significant changes in plasma expression between any two groups. Of the 399 proteins, 167 proteins had multiple unique sequences and high peptide ID confidence (>90%) and were defined as pr

SUBMITTER: Kumar S 

PROVIDER: S-EPMC4354878 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature