FTO is necessary for the induction of leptin resistance by high-fat feeding.

ABSTRACT:

Objective

Loss of function FTO mutations significantly impact body composition in humans and mice, with Fto-deficient mice reported to resist the development of obesity in response to a high-fat diet (HFD). We aimed to further explore the interactions between FTO and HFD and determine if FTO can influence the adverse metabolic consequence of HFD.

Methods

We studied mice deficient in FTO in two well validated models of leptin resistance (HFD feeding and central palmitate injection) to determine how Fto genotype may influence the action of leptin. Using transcriptomic analysis of hypothalamic tissue to identify relevant pathways affected by the loss of Fto, we combined data from co-immunoprecipitation, yeast 2-hybrid and luciferase reporter assays to identify mechanisms throug

SUBMITTER: Tung YC 

PROVIDER: S-EPMC4354923 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature