Intraindividual variation and short-term temporal trend in DNA methylation of human blood.

ABSTRACT:

Background

Between- and within-person variation in DNA methylation levels are important parameters to be considered in epigenome-wide association studies. Temporal change is one source of within-person variation in DNA methylation that has been linked to aging and disease.

Methods

We analyzed CpG-site-specific intraindividual variation and short-term temporal trend in leukocyte DNA methylation among 24 healthy Chinese women, with blood samples drawn at study entry and after 9 months. Illumina HumanMethylation450 BeadChip was used to measure methylation. Intraclass correlation coefficients (ICC) and trend estimates were summarized by genomic location and probe type.

Results

The median ICC was 0.36 across nonsex chromosomes and 0.80 on the X chromosome. There was little

SUBMITTER: Shvetsov YB 

PROVIDER: S-EPMC4355238 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature