Histology-directed and imaging mass spectrometry: An emerging technology in ectopic calcification.

ABSTRACT: The present study was designed to demonstrate the potential of an optimized histology directed protein identification combined with imaging mass spectrometry technology to reveal and identify molecules associated to ectopic calcification in human tissue. As a proof of concept, mineralized and non-mineralized areas were compared within the same dermal tissue obtained from a patient affected by Pseudoxanthoma elasticum, a genetic disorder characterized by calcification only at specific sites of soft connective tissues. Data have been technically validated on a contralateral dermal tissue from the same subject and compared with those from control healthy skin. Results demonstrate that this approach 1) significantly reduces the effects generated by techniques that, disrupting tissue organizati

SUBMITTER: Taverna D 

PROVIDER: S-EPMC4355241 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature