Global gene expression in granulosa cells of growing, plateau and atretic dominant follicles in cattle.

ABSTRACT:

Background

The physiological state of the dominant follicle is important as it may be linked to the competence of the oocyte within. The objective of this study was to analyze, by transcriptomic analysis, the changes occurring in granulosa cells from dominant follicles at different phases of follicular growth.

Methods

Granulosa cells were collected from slaughterhouse dairy cattle follicles with a diameter greater than 9 mm, and were classified at different phases of follicle growth based on flow cytometry profiles of DNA content after staining with propidium iodide. Three phases were identified based on the proportion of cells in -G1 (less than 2n DNA), G0-G1 (2n DNA) or S-M (more than 2n DNA) and follicles were thus allocated to the growing, plateau or atresia group. Betwe

SUBMITTER: Girard A 

PROVIDER: S-EPMC4355352 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature