Transcriptome analysis of ageing in uninjured human Achilles tendon.

ABSTRACT:

Introduction

The risk of tendon injury and disease increases significantly with increasing age. The aim of the study was to characterise transcriptional changes in human Achilles tendon during the ageing process in order to identify molecular signatures that might contribute to age-related degeneration.

Methods

RNA for gene expression analysis using RNA-Seq and quantitative real-time polymerase chain reaction analysis was isolated from young and old macroscopically normal human Achilles tendon. RNA sequence libraries were prepared following ribosomal RNA depletion, and sequencing was undertaken by using the Illumina HiSeq 2000 platform. Expression levels among genes were compared by using fragments per kilobase of exon per million fragments mapped. Differentially expressed g

SUBMITTER: Peffers MJ 

PROVIDER: S-EPMC4355574 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature