Discovery and analysis of pancreatic adenocarcinoma genes using cDNA microarrays.

ABSTRACT:

Aim

To study the pathogenetic processes and the role of gene expression by microarray analyses in expediting our understanding of the molecular pathophysiology of pancreatic adenocarcinoma, and to identify the novel cancer-associated genes.

Methods

Nine histologically defined pancreatic head adenocarcinoma specimens associated with clinical data were studied. Total RNA and mRNA were isolated and labeled by reverse transcription reaction with Cy5 and Cy3 for cDNA probe. The cDNA microarrays that represent a set of 4 096 human genes were hybridized with labeled cDNA probe and screened for molecular profiling analyses.

Results

Using this methodology, 184 genes were screened out for differences in gene expression level after nine couples of hybridizations. Of the 184 gene

SUBMITTER: Jin G 

PROVIDER: S-EPMC4355802 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature