Replicative senescence is associated with nuclear reorganization and with DNA methylation at specific transcription factor binding sites.

ABSTRACT:

Background

Primary cells enter replicative senescence after a limited number of cell divisions. This process needs to be considered in cell culture experiments, and it is particularly important for regenerative medicine. Replicative senescence is associated with reproducible changes in DNA methylation (DNAm) at specific sites in the genome. The mechanism that drives senescence-associated DNAm changes remains unknown - it may involve stochastic DNAm drift due to imperfect maintenance of epigenetic marks or it is directly regulated at specific sites in the genome.

Results

In this study, we analyzed the reorganization of nuclear architecture and DNAm changes during long-term culture of human fibroblasts and mesenchymal stromal cells (MSCs). We demonstrate that telomeres shorten

SUBMITTER: Hanzelmann S 

PROVIDER: S-EPMC4356053 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature