Selective pressure of antibiotics on ARGs and bacterial communities in manure-polluted freshwater-sediment microcosms.

ABSTRACT: The aim of this study was to investigate selective pressure of antibiotics on antibiotic resistance genes (ARGs) and bacterial communities in manure-polluted aquatic environment. Three treatment groups were set up in freshwater-sediment microcosms: tetracyclines group, sulfonamides group and fluoroquinolones group. Sediment and water samples were collected on day 14 after treatment. Antibiotic concentrations, ARGs abundances and bacterial community composition were analyzed. Antibiotic concentrations were determined by ultra-performance liquid chromatography-electrospray tandem mass spectrometry. ARGs abundances were quantified by real time quantitative PCR. Bacterial community composition was analyzed based on amplicon sequencing. Of the three classes of antibiotics analyzed in the treatm

SUBMITTER: Xiong W 

PROVIDER: S-EPMC4356103 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature