Age and the means of bypassing stasis influence the intrinsic subtype of immortalized human mammary epithelial cells.

ABSTRACT: Based on molecular features, breast cancers are grouped into intrinsic subtypes that have different prognoses and therapeutic response profiles. With increasing age, breast cancer incidence increases, with hormone receptor-positive and other luminal-like subtype tumors comprising a majority of cases. It is not known at what stage of tumor progression subtype specification occurs, nor how the process of aging affects the intrinsic subtype. We examined subtype markers in immortalized human mammary epithelial cell lines established following exposure of primary cultured cell strains to a two-step immortalization protocol that targets the two main barriers to immortality: stasis (stress-associated senescence) and replicative senescence. Cell lines derived from epithelial cells obtained from no

SUBMITTER: Lee JK 

PROVIDER: S-EPMC4356162 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature