Silver resistance in Gram-negative bacteria: a dissection of endogenous and exogenous mechanisms.

ABSTRACT:

Objectives

To gain a more detailed understanding of endogenous (mutational) and exogenous (horizontally acquired) resistance to silver in Gram-negative pathogens, with an emphasis on clarifying the genetic bases for resistance.

Methods

A suite of microbiological and molecular genetic techniques was employed to select and characterize endogenous and exogenous silver resistance in several Gram-negative species.

Results

In Escherichia coli, endogenous resistance arose after 6 days of exposure to silver, a consequence of two point mutations that were both necessary and sufficient for the phenotype. These mutations, in ompR and cusS, respectively conferred loss of the OmpC/F porins and derepression of the CusCFBA efflux transporter, both phenotypic changes previously linke

SUBMITTER: Randall CP 

PROVIDER: S-EPMC4356207 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature