Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging.

ABSTRACT: Signal transducers and activators of transcription (STATs) can transduce cytokine signals and regulate gene expression. The cellular localization and nuclear trafficking of STAT1, a representative of the STAT family with multiple transcriptional functions, is tightly related with transcription process, which usually happens in the interphase of the cell cycle. However, these priority questions regarding STAT1 distribution and localization at the different cell-cycle stages remain unclear. By using direct stochastic optical reconstruction microscopy (dSTORM), we found that the nuclear expression level of STAT1 increased gradually as the cell cycle carried out, especially after EGF stimulation. Furthermore, STAT1 formed clusters in the whole cell during the cell cycle, with the size and the

SUBMITTER: Gao J 

PROVIDER: S-EPMC4356954 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature