Brain region-specific gene expression profiles in freshly isolated rat microglia.

ABSTRACT: Microglia are important cells in the brain that can acquire different morphological and functional phenotypes dependent on the local situation they encounter. Knowledge on the region-specific gene signature of microglia may hold valuable clues for microglial functioning in health and disease, e.g., Parkinson's disease (PD) in which microglial phenotypes differ between affected brain regions. Therefore, we here investigated whether regional differences exist in gene expression profiles of microglia that are isolated from healthy rat brain regions relevant for PD. We used an optimized isolation protocol based on a rapid isolation of microglia from discrete rat gray matter regions using density gradients and fluorescent-activated cell sorting. Application of the present protocol followed by g

SUBMITTER: Doorn KJ 

PROVIDER: S-EPMC4357261 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature