MeRIP-PF: an easy-to-use pipeline for high-resolution peak-finding in MeRIP-Seq data.

ABSTRACT: RNA modifications, especially methylation of the N(6) position of adenosine (A)-m(6)A, represent an emerging research frontier in RNA biology. With the rapid development of high-throughput sequencing technology, in-depth study of m(6)A distribution and function relevance becomes feasible. However, a robust method to effectively identify m(6)A-modified regions has not been available yet. Here, we present a novel high-efficiency and user-friendly analysis pipeline called MeRIP-PF for the signal identification of MeRIP-Seq data in reference to controls. MeRIP-PF provides a statistical P-value for each identified m(6)A region based on the difference of read distribution when compared to the controls and also calculates false discovery rate (FDR) as a cut off to differentiate reliable m(6)A reg

SUBMITTER: Li Y 

PROVIDER: S-EPMC4357668 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature