Dynamic Escherichia coli SeqA complexes organize the newly replicated DNA at a considerable distance from the replisome.

ABSTRACT: The Escherichia coli SeqA protein binds to newly replicated, hemimethylated DNA behind replication forks and forms structures consisting of several hundred SeqA molecules bound to about 100 kb of DNA. It has been suggested that SeqA structures either direct the new sister DNA molecules away from each other or constitute a spacer that keeps the sisters together. We have developed an image analysis script that automatically measures the distance between neighboring foci in cells. Using this tool as well as direct stochastic optical reconstruction microscopy (dSTORM) we find that in cells with fluorescently tagged SeqA and replisome the sister SeqA structures were situated close together (less than about 30 nm apart) and relatively far from the replisome (on average 200-300 nm). The results s

SUBMITTER: Helgesen E 

PROVIDER: S-EPMC4357733 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature