Decreasing global transcript levels over time suggest that phytoplasma cells enter stationary phase during plant and insect colonization.

ABSTRACT: To highlight different transcriptional behaviors of the phytoplasma in the plant and animal host, expression of 14 genes of "Candidatus Phytoplasma asteris," chrysanthemum yellows strain, was investigated at different times following the infection of a plant host (Arabidopsis thaliana) and two insect vector species (Macrosteles quadripunctulatus and Euscelidius variegatus). Target genes were selected among those encoding antigenic membrane proteins, membrane transporters, secreted proteins, and general enzymes. Transcripts were detected for all analyzed genes in the three hosts; in particular, those encoding the antigenic membrane protein Amp, elements of the mechanosensitive channel, and two of the four secreted proteins (SAP54 and TENGU) were highly accumulated, suggesting that they play

SUBMITTER: Pacifico D 

PROVIDER: S-EPMC4357924 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature