High-throughput analysis of gene essentiality and sporulation in Clostridium difficile.

ABSTRACT:

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Clostridium difficile is the most common cause of antibiotic-associated intestinal infections and a significant cause of morbidity and mortality. Infection with C. difficile requires disruption of the intestinal microbiota, most commonly by antibiotic usage. Therapeutic intervention largely relies on a small number of broad-spectrum antibiotics, which further exacerbate intestinal dysbiosis and leave the patient acutely sensitive to reinfection. Development of novel targeted therapeutic interventions will require a detailed knowledge of essential cellular processes, which represent attractive targets, and species-specific processes, such as bacterial sporulation. Our knowledge of the genetic basis of C. difficile infection has been hampered by a lack of genetic tools, al

SUBMITTER: Dembek M 

PROVIDER: S-EPMC4358009 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature