Shigella flexneri regulation of ARF6 activation during bacterial entry via an IpgD-mediated positive feedback loop.

ABSTRACT:

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Entry into cells is critical for virulence of the human bacterial pathogens Shigella spp. Shigella spp. induce membrane ruffle formation and macropinocytic uptake, but the events instigating this process are incompletely understood. The host small GTPase ADP-ribosylation factor 6 (ARF6) functions in membrane trafficking at the plasma membrane and activates membrane ruffle formation. We demonstrate that ARF6 is required for efficient Shigella flexneri entry, is activated by S. flexneri dependent on the phosphatase activity of the type III secreted effector IpgD, and depends on cytohesin guanine nucleotide exchange factors (GEFs) for recruitment to entry sites. The cytohesin GEF ARF nucleotide binding site opener (ARNO) is recruited to these sites, also dependent on IpgD p

SUBMITTER: Garza-Mayers AC 

PROVIDER: S-EPMC4358011 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature