Effects of α-enolase (ENO1) over-expression on malignant biological behaviors of AGS cells.

ABSTRACT:

Objective

To investigate the effects of α-Enolase (ENO1) over-expression on the proliferative and migratory abilities of AGS cells.

Methods

The target gene was cloned and mounted to the eukaryotic expression vector pcDNA3.1(+), then was transfected into gastric cancer cell lines AGS. mRNA and protein level of ENO1 in AGS cells were verified by real-time quantitative RT-PCR and Western Blot, respectively. The effects of over-expression of ENO1 on proliferative and migratory abilities of AGS cells were detected by the experiments of CCK-8, colony formation and wound healing assays.

Results

The eukaryotic expression vector pcDNA3.1(+)/eno1 was successfully constructed, and verified by sequencing. It was shown from the cell proliferation curves that the proliferative abil

SUBMITTER: Liu YQ 

PROVIDER: S-EPMC4358447 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature