Quantitative proteomic analysis reveals a simple strategy of global resource allocation in bacteria.

ABSTRACT: A central aim of cell biology was to understand the strategy of gene expression in response to the environment. Here, we study gene expression response to metabolic challenges in exponentially growing Escherichia coli using mass spectrometry. Despite enormous complexity in the details of the underlying regulatory network, we find that the proteome partitions into several coarse-grained sectors, with each sector's total mass abundance exhibiting positive or negative linear relations with the growth rate. The growth rate-dependent components of the proteome fractions comprise about half of the proteome by mass, and their mutual dependencies can be characterized by a simple flux model involving only two effective parameters. The success and apparent generality of this model arises from tight

SUBMITTER: Hui S 

PROVIDER: S-EPMC4358657 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature