Cell type specific gene expression analysis of prostate needle biopsies resolves tumor tissue heterogeneity.

ABSTRACT: A lack of cell surface markers for the specific identification, isolation and subsequent analysis of living prostate tumor cells hampers progress in the field. Specific characterization of tumor cells and their microenvironment in a multi-parameter molecular assay could significantly improve prognostic accuracy for the heterogeneous prostate tumor tissue. Novel functionalized gold-nano particles allow fluorescence-based detection of absolute mRNA expression levels in living cells by fluorescent activated flow cytometry (FACS). We use of this technique to separate prostate tumor and benign cells in human prostate needle biopsies based on the expression levels of the tumor marker alpha-methylacyl-CoA racemase (AMACR). We combined RNA and protein detection of living cells by FACS to gate for

SUBMITTER: Kronig M 

PROVIDER: S-EPMC4359234 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature