Molecular epidemiological study of feline coronavirus strains in Japan using RT-PCR targeting nsp14 gene.

ABSTRACT:

Background

Feline infectious peritonitis is a fatal disease of cats caused by infection with feline coronavirus (FCoV). For detecting or genotyping of FCoV, some RT-PCR plus nested PCR techniques have been reported previously. However, referring to the whole genome sequences (WGSs) registered at NCBI, there are no detection methods that can tolerate the genetic diversity among FCoV population. In addition, the quasispecies nature of FCoV, which consists of heterogeneous variants, has been also demonstrated; thus, a universal method for heteropopulations of FCoV variants in clinical specimens is desirable.

Results

To develop an RT-PCR method for detection and genotyping of FCoV, we performed comparative genome analysis using WGSs of 32 FCoV, 7 CCoV and 5 TGEV strains obtained

SUBMITTER: Tanaka Y 

PROVIDER: S-EPMC4359392 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature