Analyzing insulin samples by size-exclusion chromatography: a column degradation study.

ABSTRACT: Investigating insulin analogs and probing their intrinsic stability at physiological temperature, we observed significant degradation in the size-exclusion chromatography (SEC) signal over a moderate number of insulin sample injections, which generated concerns about the quality of the separations. Therefore, our research goal was to identify the cause(s) for the observed signal degradation and attempt to mitigate the degradation in order to extend SEC column lifespan. In these studies, we used multiangle light scattering, nuclear magnetic resonance, and gas chromatography-mass spectrometry methods to evaluate column degradation. The results from these studies illustrate: (1) that zinc ions introduced by the insulin product produced the observed column performance issues; and (2) that incl

SUBMITTER: Teska BM 

PROVIDER: S-EPMC4359628 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature