A simple flow cytometry method improves the detection of phosphatidylserine-exposing extracellular vesicles.

ABSTRACT:

Background

Plasma contains cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential applications as disease biomarker. However, the enumeration of EVs faces major problems, due to their sub-micrometer size and to intrinsic limitations in methods of characterization, mainly flow cytometry (FCM).

Objectives

Our objective is to enumerate EVs in plasma, by taking as the prototype the population of phosphatidylserine (PS)-exposing EVs, which constitute one of the major EV populations and are responsible for thrombotic disorders.

Methods

The concentration of PS-exposing EVs in platelet-free plasma (PFP) of healthy subjects was measured by FCM using either light scattering or fluorescence as the trigger and fluorescent A

SUBMITTER: Arraud N 

PROVIDER: S-EPMC4359678 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature