Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy.

ABSTRACT:

Background

The DHFR negative CHO DXB11 cell line (also known as DUX-B11 and DUKX) was historically the first CHO cell line to be used for large scale production of heterologous proteins and is still used for production of a number of complex proteins.

Results

Here we present the genomic sequence of the CHO DXB11 genome sequenced to a depth of 33x. Overall a significant genomic drift was seen favoring GC → AT point mutations in line with the chemical mutagenesis strategy used for generation of the cell line. The sequencing depth for each gene in the genome revealed distinct peaks at sequencing depths of 0x, 16x, 33x and 49x coverage corresponding to a copy number in the genome of 0, 1, 2 and 3 copies. This indicate that 17% of the genes are haploid revealing a large number of

SUBMITTER: Kaas CS 

PROVIDER: S-EPMC4359788 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature