Identification and genetic characterization by high-throughput SNP analysis of intervarietal substitution lines of rapeseed (Brassica napus L.) with enhanced embryogenic potential.

ABSTRACT:

Key message

Seven intervarietal substitution lines were identified with embryogenic potentials up to 40.4 times that of the recurrent parent, providing an ideal material for further in depth studies of this trait. To identify genomic regions that carry genetic factors controlling embryogenic potential of isolated microspores of rapeseed, marker segregations were analysed in a segregating population of haploid microspore-derived embryos and a BC1 population from a cross between 'Express 617' and 'RS239'. After map construction 15 intervarietal substitution lines from the same cross with 'Express 617' as recurrent parent were selected with donor segments covering five genomic regions that had shown skewed segregations in the population of microspore-derived embryos but not in the BC1

SUBMITTER: Ecke W 

PROVIDER: S-EPMC4361729 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature