Genetic rearrangements of variable di-residue (RVD)-containing repeat arrays in a baculoviral TALEN system.

ABSTRACT: Virus-derived gene transfer vectors have been successfully employed to express the transcription activator-like effector nucleases (TALENs) in mammalian cells. Since the DNA-binding domains of TALENs consist of the variable di-residue (RVD)-containing tandem repeat modules and virus genome with repeated sequences is susceptible to genetic recombination, we investigated several factors that might affect TALEN cleavage efficiency of baculoviral vectors. Using a TALEN system designed to target the AAVS1 locus, we observed increased sequence instability of the TALE repeat arrays when a higher multiplicity of infection (MOI) of recombinant viruses was used to produce the baculoviral vectors. We also detected more deleterious mutations in the TALE DNA-binding domains when both left and right TAL

SUBMITTER: Lau CH 

PROVIDER: S-EPMC4362386 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature