CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells.

ABSTRACT:

Background

The histone H3 variant CENP-A is normally tightly regulated to ensure only one centromere exists per chromosome. Native CENP-A is often found overexpressed in human cancer cells and a range of human tumors. Consequently, CENP-A misregulation is thought to contribute to genome instability in human cancers. However, the consequences of such overexpression have not been directly elucidated in human cancer cells.

Results

To investigate native CENP-A overexpression, we sought to uncover CENP-A-associated defects in human cells. We confirm that CENP-A is innately overexpressed in several colorectal cancer cell lines. In such cells, we report that a subset of structurally distinct CENP-A-containing nucleosomes associate with canonical histone H3, and with the transcripti

SUBMITTER: Athwal RK 

PROVIDER: S-EPMC4363203 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature