MicroRNA profiling in three main stages during porcine spermatogenesis.

ABSTRACT:

Background

Spermatogenesis is an intricate biological event wherein an undifferentiated spermatogonium develops into mature sperms. MicroRNAs are a type of single strand small non-coding RNA molecule and are implicated in the regulation of many crucial pathways during cell proliferation, apoptosis, and differentiation.

Method

Here, we present a comprehensive comparison of miRNA expression profiling in three main stages during porcine spermatogenesis using high-throughput sequencing.

Results

We built three small RNA libraries for the testis, the epididymis and the ejaculated sperm from a Landrace boar, and in total obtained 3821 precursor hairpins encoding for 4761 mature miRNAs, of which 23 are miRNA*. Notably, 940 precursor miRNAs produced both the 5'- and 3'- strand

SUBMITTER: Luo Z 

PROVIDER: S-EPMC4363228 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature