Ion Torrent sequencing as a tool for mutation discovery in the flax (Linum usitatissimum L.) genome.

ABSTRACT:

Background

Detection of induced mutations is valuable for inferring gene function and for developing novel germplasm for crop improvement. Many reverse genetics approaches have been developed to identify mutations in genes of interest within a mutagenized population, including some approaches that rely on next-generation sequencing (e.g. exome capture, whole genome resequencing). As an alternative to these genome or exome-scale methods, we sought to develop a scalable and efficient method for detection of induced mutations that could be applied to a small number of target genes, using Ion Torrent technology. We developed this method in flax (Linum usitatissimum), to demonstrate its utility in a crop species.

Results

We used an amplicon-based approach in which DNA samples fro

SUBMITTER: Galindo-Gonzalez L 

PROVIDER: S-EPMC4363359 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature