An underlying mechanism for the increased mutagenesis of lagging-strand genes in Bacillus subtilis.

ABSTRACT: We previously reported that lagging-strand genes accumulate mutations faster than those encoded on the leading strand in Bacillus subtilis. Although we proposed that orientation-specific encounters between replication and transcription underlie this phenomenon, the mechanism leading to the increased mutagenesis of lagging-strand genes remained unknown. Here, we report that the transcription-dependent and orientation-specific differences in mutation rates of genes require the B. subtilis Y-family polymerase, PolY1 (yqjH). We find that without PolY1, association of the replicative helicase, DnaC, and the recombination protein, RecA, with lagging-strand genes increases in a transcription-dependent manner. These data suggest that PolY1 promotes efficient replisome progression through lagging-s

SUBMITTER: Million-Weaver S 

PROVIDER: S-EPMC4364195 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature