Evaluation of a low-density hydrogel microarray technique for mycobacterial species identification.

ABSTRACT: In addition to the obligatory pathogenic species of the Mycobacterium tuberculosis complex and Mycobacterium leprae, the genus Mycobacterium also includes conditionally pathogenic species that in rare cases can lead to the development of nontuberculous mycobacterial diseases. Because tuberculosis and mycobacteriosis have similar clinical signs, the accurate identification of the causative agent in a clinical microbiology laboratory is important for diagnostic verification and appropriate treatment. This report describes a low-density hydrogel-based microarray containing oligonucleotide probes based on the species-specific sequences of the gyrB gene fragment for mycobacterial species identification. The procedure included the amplification of a 352-nucleotide fragment of the gene and its hy

SUBMITTER: Zimenkov DV 

PROVIDER: S-EPMC4365248 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature