Mir-34b/c and mir-449a/b/c are required for spermatogenesis, but not for the first cleavage division in mice.

ABSTRACT: Mammalian sperm are carriers of not only the paternal genome, but also the paternal epigenome in the forms of DNA methylation, retained histones and noncoding RNAs. Although paternal DNA methylation and histone retention sites have been correlated with protein-coding genes that are critical for preimplantation embryonic development, physiological evidence of an essential role of these epigenetic marks in fertilization and early development remains lacking. Two miRNA clusters consisting of five miRNAs (miR-34b/c and miR-449a/b/c) are present in sperm, but absent in oocytes, and miR-34c has been reported to be essential for the first cleavage division in vitro. Here, we show that both miR-34b/c- and miR-449-null male mice displayed normal fertility, and that intracytoplasmic injection of eit

SUBMITTER: Yuan S 

PROVIDER: S-EPMC4365490 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature