Up-regulation of microRNA-21 mediates isoflurane-induced protection of cardiomyocytes.

ABSTRACT:

Background

Anesthetic cardioprotection reduces myocardial infarct size after ischemia-reperfusion injury. Currently, the role of microRNA in this process remains unknown. MicroRNAs are short, noncoding nucleotide sequences that negatively regulate gene expression through degradation or suppression of messenger RNA. In this study, the authors uncovered the functional role of microRNA-21 (miR-21) up-regulation after anesthetic exposure.

Methods

MicroRNA and messenger RNA expression changes were analyzed by quantitative real-time polymerase chain reaction in cardiomyocytes after exposure to isoflurane. Lactate dehydrogenase release assay and propidium iodide staining were conducted after inhibition of miR-21. miR-21 target expression was analyzed by Western blot. The functional

SUBMITTER: Olson JM 

PROVIDER: S-EPMC4366274 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature