In vitro differentiation of rat mesenchymal stem cells to hepatocyte lineage.

ABSTRACT:

Objectives

Mesenchyme is a type of undifferentiated loose connective tissue that is derived mostly from mesoderm. Recently, mesenchymal stem cells (MSCs), as adult stem cells (ASCs) able to divide into a variety of different cells, are of utmost importance for stem cell research. In this research, ability of the liver extract to induce differentiation of rat derived omentum tissue mesenchymal stem cells (rOT-MSCs) into hepatocyte cells (HCs) was investigated.

Materials and methods

After isolation and confirmation of rOT-MSCs they were co-cultured with liver extract and hepatogenic differentiation was monitored. Expressions of mesenchymal stem cell markers were also analyzed via flow cytometry. Moreover, expressions of octamer-binding transcription factor-4 (Oct-4), Wilm's tu

SUBMITTER: Sarvandi SS 

PROVIDER: S-EPMC4366749 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature