Selection of reference genes for gene expression studies related to intramuscular fat deposition in Capra hircus skeletal muscle.

ABSTRACT: The identification of suitable reference genes is critical for obtaining reliable results from gene expression studies using quantitative real-time PCR (qPCR) because the expression of reference genes may vary considerably under different experimental conditions. In most cases, however, commonly used reference genes are employed in data normalization without proper validation, which may lead to incorrect data interpretation. Here, we aim to select a set of optimal reference genes for the accurate normalization of gene expression associated with intramuscular fat (IMF) deposition during development. In the present study, eight reference genes (PPIB, HMBS, RPLP0, B2M, YWHAZ, 18S, GAPDH and ACTB) were evaluated by three different algorithms (geNorm, NormFinder and BestKeeper) in two types of

SUBMITTER: Zhu W 

PROVIDER: S-EPMC4368700 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature