Identification of reference genes for quantitative expression analysis of microRNAs and mRNAs in barley under various stress conditions.

ABSTRACT: For accurate and reliable gene expression analysis using quantitative real-time reverse transcription PCR (qPCR), the selection of appropriate reference genes as an internal control for normalization is crucial. We hypothesized that non-coding, small nucleolar RNAs (snoRNAs)would be stably expressed in different barley varieties and under different experimental treatments,in different tissues and at different developmental stages of plant growth and therefore might prove to be suitable reference genes for expression analysis of both microRNAs (miRNAs)and mRNAs. In this study, we examined the expression stability of ten candidate reference genes in six barley genotypes under five experimental stresses, drought, fungal infection,boron toxicity, nutrient deficiency and salinity. We compared f

SUBMITTER: Ferdous J 

PROVIDER: S-EPMC4368757 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature