Proteomic analysis of urine exosomes reveals renal tubule response to leptospiral colonization in experimentally infected rats.

ABSTRACT:

Background

Infectious Leptospira colonize the kidneys of reservoir (e.g. rats) and accidental hosts such as humans. The renal response to persistent leptospiral colonization, as measured by urinary protein biosignatures, has not been systematically studied. Urinary exosomes--bioactive membrane-bound nanovesicles--contain cell-state specific cargo that additively reflect formation all along the nephron. We hypothesized that Leptospira-infection will alter the content of urine exosomes, and further, that these Leptospira-induced alterations will hold clues to unravel novel pathways related to bacterial-host interactions.

Methodology/principal findings

Exosome protein content from 24 hour urine samples of Leptospira-infected rats was compared with that of uninfected rats using

SUBMITTER: RamachandraRao SP 

PROVIDER: S-EPMC4368819 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature